Anti-Smoothened (phospho Ser611 + Ser615 + Ser616) Antibody (A308593)

Name: Anti-Smoothened (phospho Ser611 + Ser615 + Ser616) Antibody
Brand: Antibodies.com
SKU: A308593
Price: 435.0 USD
Availability: InStock

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$435

Rabbit polyclonal antibody to Smoothened (phospho Ser611 + Ser615 + Ser616) for WB.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-Smoothened (phospho Ser611 + Ser615 + Ser616) Antibody

Description

Rabbit polyclonal antibody to Smoothened (phospho Ser611 + Ser615 + Ser616).

Applications

Dilutions

WB: 1:500-1:1,000

Reactivity

Human

Immunogen

A synthetic phosphorylated peptide around S611 & S615 & S616 of human SMO (NP_005622.1).

Sequence

SADVSS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

80 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Protein Gx, Protein smoothened, SMO, SMOH

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (1)

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Western Blot - Anti-Smoothened (phospho Ser611 + Ser615 + Ser616) Antibody (A308593)

Western blot analysis of extracts of 293T cells, using Anti-Smoothened (phospho Ser611 + Ser615 + Ser616) Antibody (A308593) at 1:1,000 dilution. 293T-SMO cells were treated by SHH (0. 4ug/ml) at 37°C after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 1min.

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