Synthetic peptide within amino acids 1175 and the C-terminus of human SMC1A (NP_006297. 2).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Tris-Citrate/Phosphate Buffer, pH 7-8, with 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
General Notes
This antibody was affinity purified using the immunising peptide immobilized on solid support. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1. 4 equals 1.0 mg of IgG.
Synonyms
DXS423E, KIAA0178, SB1.8, SMC protein 1A, SMC-1-alpha, SMC-1A, SMC1, SMC1L1, Structural maintenance of chromosomes protein 1A
Sample: FFPE section of mouse colon carcinoma CT26. Antibody: Anti-SMC1A Antibody (A295225) was used at a dilution of 1:5,000 (0.2 µg/ml). Detection: DAB.
Sample: FFPE section of human ovarian carcinoma. Antibody: Anti-SMC1A Antibody (A295225) was used at a dilution of 1:5,000 (0.2 µg/ml). Detection: DAB.
Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Anti-SMC1A Antibody (A295225) was used for IP at 6 µg per reaction. SMC1A was also immunoprecipitated with a rabbit Anti-SMC1A recombinant monoclonal antibody. For blotting immunoprecipitated SMC1A, the Anti-SMC1A recombinant monoclonal antibody was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.
Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Anti-SMC1A Antibody (A295225) was used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
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