Anti-SMAD5 (phospho Ser463 + Ser465) Antibody [ARC1568] (A308812)

Name: Anti-SMAD5 (phospho Ser463 + Ser465) Antibody [ARC1568]
Brand: Antibodies.com
SKU: A308812
Price: 560.0 USD
Availability: InStock

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$560

Rabbit monoclonal [ARC1568] antibody to SMAD5 (phospho Ser463 + Ser465) for WB.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-SMAD5 (phospho Ser463 + Ser465) Antibody [ARC1568]

Description

Rabbit monoclonal [ARC1568] antibody to SMAD5 (phospho Ser463 + Ser465).

Applications

Dilutions

WB: 1:500-1:2,000

Reactivity

Human

Immunogen

A synthetic phosphorylated peptide around S463 & S465 of human Smad5 (Q99717).

Sequence

SSVS

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1568

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

52 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

hSmad5, JV5-1, MAD homolog 5, MADH5, Mothers against decapentaplegic homolog 5, Mothers against DPP homolog 5, SMAD 5, SMAD family member 5

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (1)

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Western Blot - Anti-SMAD5 (phospho Ser463 + Ser465) Antibody [ARC1568] (A308812)

Western blot analysis of extracts of HeLa cells, using Anti-SMAD5 (phospho Ser463 + Ser465) Antibody [ARC1568] (A308812) at 1:1,000 dilution or Smad5 antibody (A1947). Hela cells were treated by ATP(5 mM) at 30°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 3min.

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