Western Blot - Anti-SMAD5 Antibody [ARC55344] (A309477)
Western blot analysis of extracts from wild type(WT) and Smad5 knockout (KO) 293T(KO) cells, using Anti-SMAD5 Antibody [ARC55344] (A309477) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
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