Recombinant Anti-Smad4 Antibody [rSMAD4/6310] (A315653)

Anti-Smad4 Antibody [rSMAD4/6310]

Recombinant mouse monoclonal [rSMAD4/6310] antibody to Smad4.

Signaling from the ligand-activated membrane receptor serine/threonine kinases to nuclear targets is mediated by a set of evolutionarily conserved proteins known as DPC4.Upon ligand binding, the receptors of the TGF- family phosphorylate SMAD proteins (SMAD1 and SMAD2). These proteins then move into the nucleus, where they activate transcription. To carry out this function, the receptor activated SMAD1 and 2 require association with the product of deleted in pancreatic carcinoma, locus 4 (DPC4), also known as SMAD4. SMAD4/DPC4 is also implicated as a tumor suppressor, since it is inactivated in more than half of pancreatic carcinomas and to a lesser extent in a variety of other cancers. The lack of SMAD4 expression is present in approximately 80% of cases of pancreatic adenocarcinoma, but rarely in endometrial (0%), colorectal (0%), ovarian (3%), lung (0%), breast (2%) adenocarcinomas, and malignant melanoma (4%). SMAD4is an important marker for confirming a diagnosis of pancreatic adenocarcinoma.

Human pancreatic adenocarcinoma.

IHC: 1-2 µg/ml

Human, Mouse,, Rat

Amino acids 1-552 representing full-length human SMAD4.

Mouse

Monoclonal

rSMAD4/6310

kappa

Protein A/G chromatography.

200 µg/ml

61 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Deletion target in pancreatic carcinoma 4, DPC4, hSMAD4, MAD homolog 4, MADH4, Mothers against decapentaplegic homolog 4, Mothers against DPP homolog 4, SMAD 4, SMAD family member 4