Anti-Smad4 Antibody [ARC5009-06] (A308139)

Name: Anti-Smad4 Antibody [ARC5009-06]
Brand: Antibodies.com
SKU: A308139
Price: 470.0 USD
Availability: InStock

Enlarge Image

$470

Rabbit monoclonal [ARC5009-06] antibody to Smad4 for WB, IHC, IP and ChIP.

100% Guarantee

Price Match Guarantee

Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Tags:

Knockout Validated Antibody

Specifications

Name

Anti-Smad4 Antibody [ARC5009-06]

Description

Rabbit monoclonal [ARC5009-06] antibody to Smad4.

Applications

WB, IHC, IP, ChIP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, IP: 1:500-1:1,000, ChIP: 1:500-1:1,000, CUT&Tag: 1⁶ cells/1µg

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 320-552 of human Smad4 (NP_005350.1).

Sequence

PEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLSAAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQPLD

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC5009-06

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

65 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Deletion target in pancreatic carcinoma 4, DPC4, hSMAD4, MAD homolog 4, MADH4, Mothers against decapentaplegic homolog 4, Mothers against DPP homolog 4, SMAD 4, SMAD family member 4

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (6)

Enlarge Image

Anti-Smad4 Antibody [ARC5009-06] (A308139)

CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina from 10⁵ K562 cells with 1µg of Anti-Smad4 Antibody [ARC5009-06] (A308139), and Goat Anti-Rabbit IgG H&L Antibody. The CUT&Tag results indicate the enrichment pattern of Smad4 in representative gene loci (JUNB), as shown in figure.

Enlarge Image

Western Blot - Anti-Smad4 Antibody [ARC5009-06] (A308139)

Western blot analysis of extracts of various cell lines, using Anti-Smad4 Antibody [ARC5009-06] (A308139) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Enlarge Image

Western Blot - Anti-Smad4 Antibody [ARC5009-06] (A308139)

Enlarge Image

Immunohistochemistry - Anti-Smad4 Antibody [ARC5009-06] (A308139)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-Smad4 Antibody [ARC5009-06] (A308139) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.