Anti-Smad3 (Phospho-Ser425) Antibody (A50434)

Name: Anti-Smad3 (Phospho-Ser425) Antibody
Brand: Antibodies.com
SKU: A50434
Price: 275.0 USD
Availability: InStock

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$275

Rabbit polyclonal antibody to Smad3 (Phospho-Ser425) for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 6-9 business days.

Specifications

Name

Anti-Smad3 (Phospho-Ser425) Antibody

Description

Rabbit polyclonal antibody to Smad3 (Phospho-Ser425)

Specificity

The antibody detects endogenous level of Smad3 only when phosphorylated at serine 425.

Applications

WB, IHC

Reactivity

Human, Mouse, Rat

Immunogen

Peptide sequence around phosphorylation site of serine 425 (C-S-S-V-S(p)) derived from Human Smad3.

Host

Rabbit

Clonality

Polyclonal

Conjugate

Unconjugated

Purification

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/ml

Formulation

Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Synonyms

hMAD-3, hSMAD3, JV15-2, MAD homolog 3, Mad3, MADH3, Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMAD 3, SMAD family member 3

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Anti-Smad3 (Phospho-Ser425) Antibody (A50434)

Western blot analysis of extracts from Hela cells untreated or treated with EGF using Smad3 (Phospho-Ser425) Antibody #11325.

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Immunohistochemistry - Anti-Smad3 (Phospho-Ser425) Antibody (A50434)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Smad3 (Phospho-Ser425) Antibody #11325 (left) or the same antibody preincubated with blocking peptide (right).

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