Western Blot - Anti-Smad2 (phospho Ser467) Antibody (A16558)
Western blot analysis of extracts of HeLa cells, using Anti-Smad2 (phospho Ser467) Antibody (A16558) at 1:1,000 dilution. Hela cells were treated by TGF-beta (10 ng/ml) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
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