Anti-Smad2 (phospho Ser250) Antibody [ARC1552] (A305752)

Name: Anti-Smad2 (phospho Ser250) Antibody [ARC1552]
Brand: Antibodies.com
SKU: A305752
Price: 560.0 USD
Availability: InStock

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$560

Rabbit monoclonal [ARC1552] antibody to Smad2 (phospho Ser250) for WB.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-Smad2 (phospho Ser250) Antibody [ARC1552]

Description

Rabbit monoclonal [ARC1552] antibody to Smad2 (phospho Ser250).

Applications

Dilutions

WB: 1:500-1:2,000

Reactivity

Human, Mouse

Immunogen

A synthetic phosphorylated peptide around S250 of human Phospho-Smad2-S250 (Q15796).

Sequence

ELSPT

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1552

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

58 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

hMAD-2, hSMAD2, JV18-1, MAD homolog 2, Mad-related protein 2, MADH2, MADR2, Mothers against decapentaplegic homolog 2, Mothers against DPP homolog 2, SMAD 2, SMAD family member 2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (1)

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Western Blot - Anti-Smad2 (phospho Ser250) Antibody [ARC1552] (A305752)

Western blot analysis of extracts of various cell lines, using Anti-Smad2 (phospho Ser250) Antibody [ARC1552] (A305752) at 1:1,000 dilution. 293T cells were treated by PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight or treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. C2C12 cells were treated by PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 10s.

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