Anti-Smad2 (phospho Ser245 + Ser250 + Ser255) Antibody [ARC53851] (A308749)

Name: Anti-Smad2 (phospho Ser245 + Ser250 + Ser255) Antibody [ARC53851]
Brand: Antibodies.com
SKU: A308749
Price: 560.0 USD
Availability: InStock

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$560

Rabbit monoclonal [ARC53851] antibody to Smad2 (phospho Ser245 + Ser250 + Ser255) for WB.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-Smad2 (phospho Ser245 + Ser250 + Ser255) Antibody [ARC53851]

Description

Rabbit monoclonal [ARC53851] antibody to Smad2 (phospho Ser245 + Ser250 + Ser255).

Applications

Dilutions

WB: 1:1,000-1:5,000

Reactivity

Human

Immunogen

A synthetic phosphorylated peptide around S245 & S250 & S255 of human Smad2.

Sequence

SPAELSPTTLS

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC53851

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

hMAD-2, hSMAD2, JV18-1, MAD homolog 2, Mad-related protein 2, MADH2, MADR2, Mothers against decapentaplegic homolog 2, Mothers against DPP homolog 2, SMAD 2, SMAD family member 2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (1)

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Western Blot - Anti-Smad2 (phospho Ser245 + Ser250 + Ser255) Antibody [ARC53851] (A308749)

Western blot analysis of various lysates, using Anti-Smad2 (phospho Ser245 + Ser250 + Ser255) Antibody [ARC53851] (A308749) at 1:2,000 dilution. 293T cells were treated by PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.

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