Anti-SIRT4 Antibody (A12140)

Name: Anti-SIRT4 Antibody
Brand: Antibodies.com
SKU: A12140
Price: 355.0 USD
Availability: InStock

Enlarge Image

$355

Rabbit polyclonal antibody to SIRT4 for WB and IHC.

100% Guarantee

Price Match Guarantee

Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-SIRT4 Antibody

Description

Rabbit polyclonal antibody to SIRT4.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 29-120 of human SIRT4 (NP_036372.1).

Sequence

IGLFVPASPPLDPEKVKELQRFITLSKRLLVMTGAGISTESGIPDYRSEKVGLYARTDRRPIQHGDFVRSAPIRQRYWARNFVGWPQFSSHQ

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

32 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

NAD-dependent ADP-ribosyltransferase sirtuin-4, NAD-dependent protein biotinylase sirtuin-4, NAD-dependent protein deacetylase sirtuin-4, NAD-dependent protein lipoamidase sirtuin-4, mitochondrial, Regulatory protein SIR2 homolog 4, SIR2-like protein 4, SIR2L4

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

Enlarge Image

Western Blot - Anti-SIRT4 Antibody (A12140)

Western blot analysis of extracts of various cell lines, using Anti-SIRT4 Antibody (A12140) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

Enlarge Image

Immunohistochemistry - Anti-SIRT4 Antibody (A12140)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-SIRT4 Antibody (A12140) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Enlarge Image

Immunohistochemistry - Anti-SIRT4 Antibody (A12140)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-SIRT4 Antibody (A12140) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Enlarge Image

Immunohistochemistry - Anti-SIRT4 Antibody (A12140)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-SIRT4 Antibody (A12140) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Publishing research using Anti-SIRT4 Antibody (A12140)? Please let us know so that we can list the citation on this page.