Anti-SIRT2 Antibody (A12592)

Name: Anti-SIRT2 Antibody
Brand: Antibodies.com
SKU: A12592
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to SIRT2 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-SIRT2 Antibody

Description

Rabbit polyclonal antibody to SIRT2.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 288-389 of human SIRT2 (NP_036369.2).

Sequence

EKAGQSDPFLGMIMGLGGGMDFDSKKAYRDVAWLGECDQGCLALAELLGWKKELEDLVRREHASIDAQSGAGVPNPSTSASPKKSPPPAKDEARTTEREKPQ

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

39 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

NAD-dependent protein deacetylase sirtuin-2, NAD-dependent protein defatty-acylase sirtuin-2, Regulatory protein SIR2 homolog 2, SIR2-like protein 2, SIR2L, SIR2L2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-SIRT2 Antibody (A12592)

Western blot analysis of various lysates, using Anti-SIRT2 Antibody (A12592) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Immunohistochemistry - Anti-SIRT2 Antibody (A12592)

Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-SIRT2 Antibody (A12592) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-SIRT2 Antibody (A12592)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-SIRT2 Antibody (A12592) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-SIRT2 Antibody (A12592)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-SIRT2 Antibody (A12592) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.