Rabbit monoclonal [ARC1520] antibody to SIRT1 (phospho Ser47).
Applications
WB
Dilutions
WB: 1:100-1:500
Reactivity
Human, Mouse
Immunogen
A synthetic phosphorylated peptide around S47 of human SIRT1 (Q96EB6).
Sequence
ERSPG
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC1520
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity purification.
Molecular Weight
120 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
hSIR2, hSIRT1, NAD-dependent protein deacetylase sirtuin-1, NAD-dependent protein deacylase sirtuin-1, Regulatory protein SIR2 homolog 1, SIR2-like protein 1, SIR2L1
Western Blot - Anti-SIRT1 (phospho Ser47) Antibody [ARC1520] (A305791)
Western blot analysis of extracts of 293T cells, using Anti-SIRT1 (phospho Ser47) Antibody [ARC1520] (A305791) at 1:500 dilution. 293T cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
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