Anti-SIRT1 Antibody (A91756)

Name: Anti-SIRT1 Antibody
Brand: Antibodies.com
SKU: A91756
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to SIRT1 for WB, ICC/IF, IP and ChIP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Tags:

Nuclear Marker

Specifications

Name

Anti-SIRT1 Antibody

Description

Rabbit polyclonal antibody to SIRT1.

Applications

WB, ICC/IF, IP, ChIP

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200, IP: 50-1:200, ChIP: 1:20-1:100

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 448-747 of human SIRT1 (NP_036370.2).

Sequence

VALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRTQKELAYLSELPPTPLHVSEDSSSPERTSPPDSSVIVTLLDQAAKSNDDLDVSESKGCMEEKPQEVQTSRNVESIAEQMENPDLKNVGSSTGEKNERTSVAGTVRKCWPNRVAKEQISRRLDGNQYLFLPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPMEDESEIEEFYNGLEDEPDVPERAGGAGFGTDGDDQEAINEAISVKQEVTDMNYPSNKS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

120 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

hSIR2, hSIRT1, NAD-dependent protein deacetylase sirtuin-1, NAD-dependent protein deacylase sirtuin-1, Regulatory protein SIR2 homolog 1, SIR2-like protein 1, SIR2L1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-SIRT1 Antibody (A91756)

Western blot analysis of extracts of various cell lines, using Anti-SIRT1 Antibody (A91756) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Immunofluorescence - Anti-SIRT1 Antibody (A91756)

Immunofluorescence analysis of HeLa cells using Anti-SIRT1 Antibody (A91756) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-SIRT1 Antibody (A91756)

Immunofluorescence analysis of NIH/3T3 cells using Anti-SIRT1 Antibody (A91756) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).

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