Synthetic peptide corresponding to the internal region, amino acids 577-590, of human SIRT1.
Sequence
C-EKPQEVQTSRNVES
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Molecular Weight
120 kDa
Predicted MW
81.7kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
hSIR2, hSIRT1, NAD-dependent protein deacetylase sirtuin-1, NAD-dependent protein deacylase sirtuin-1, Regulatory protein SIR2 homolog 1, SIR2-like protein 1, SIR2L1
SIRT1 expression in Jurkat cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-SIRT1 Antibody (A82815) at 0.05µg/ml and detected by chemiluminescence.
SIRT1 expression in Human Skin analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-SIRT1 Antibody (A82815) at 3.8µg/ml and revealed with alkaline phosphatase (AP).
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