Anti-SIRP alpha Antibody [DH59B] (A280253)

Anti-SIRP alpha Antibody [DH59B]

Mouse monoclonal [DH59B] antibody to SIRP alpha.

This antibody recognises bovine CD172a also known as SIRP alpha or MyD-1 antigen. CD172a is a transmembrane signal regulatory protein expressed primarily by macrophages, monocytes, dendritic cells, granulocytes, myeloid progenitors, hematopoietic stem cells, and neurons. The extracellular region of SIRP family consists of three immunoglobulin superfamily (IgSF) domains; two IgC and one IgV domain. The IgV domain of CD172a binds to CD47. The binding domain of CD172a is analogous to that of immunoglobulins and T cell receptors and is involved in myeloid cell activation. However, signaling via CD172a is mainly inhibitory to cell function and phagocytosis. CD172a expressing cells are stimulated in Mycobacterium tuberculosis infection, influencing migration of dendritic cells and macrophages, phagocytosis, and granuloma formation.

IHC-Fr, Flow Cytometry

Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Bovine, Bison, Water Buffalo, Sheep, Horse, Dog, Cat

Cells from multiple species with the final screening of the fusion on cells from dog.

Mouse

Monoclonal

DH59B

IgG1

1 mg/ml

Liquid

Supplied in serum free tissue culture medium containing proprietary protein free supplement and 0.09% Sodium Azide.

Store at 4°C. Do not freeze! Should this product contain a precipitate we recommend microcentrifugation before use.

Mouse anti Bovine CD172a, clone DH59B, recognizes bovine CD172a also known as SIRP alpha or MyD-1 antigen. CD172a is a transmembrane signal regulatory protein expressed primarily by macrophages, monocytes, dendritic cells, granulocytes, myeloid progenitors, hematopoietic stem cells, and neurons (Barclay et al. 2006). The extracellular region of SIRP family consists of three immunoglobulin superfamily (IgSF) domains; two IgC and one IgV domain (Barclay et al. 2006). The IgV domain of CD172a binds to CD47 (Hatherley et al. 2007).The binding domain of CD172a is analogous to that of immunoglobulins and T cell receptors and is involved in myeloid cell activation (Berg et al.2004). However, signaling via CD172a is mainly inhibitory to cell function and phagocytosis (Oldenborg et al. 2001). CD172a expressing cells are stimulated in Mycobacterium tuberculosis infection, influencing migration of dendritic cells and macrophages, phagocytosis, and granuloma formation (Waters et al. 2009).

BIT, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, CD172a, Inhibitory receptor SHPS-1, Macrophage fusion receptor, MFR, MyD-1 antigen, MYD1, PTPNS1, SHP substrate 1, SHPS-1, SHPS1, SIRP, SIRPA, Tyrosine-protein phosphatase non-receptor type substrate 1