Anti-SHP2 Antibody (A93012)

Name: Anti-SHP2 Antibody
Brand: Antibodies.com
SKU: A93012
Price: 485.0 USD
Availability: InStock

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$485

Rabbit polyclonal antibody to SHP2 for WB, ICC/IF and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Tags:

Knockout Validated Antibody

Specifications

Name

Anti-SHP2 Antibody

Description

Rabbit polyclonal antibody to SHP2.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200, IP: 1:1,000-1:5,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-460 of human SHP2 (NP_002825.3).

Sequence

MTSRRWFHPNITGVEAENLLLTRGVDGSFLARPSKSNPGDFTLSVRRNGAVTHIKIQNTGDYYDLYGGEKFATLAELVQYYMEHHGQLKEKNGDVIELKYPLNCADPTSERWFHGHLSGKEAEKLLTEKGKHGSFLVRESQSHPGDFVLSVRTGDDKGESNDGKSKVTHVMIRCQELKYDVGGGERFDSLTDLVEHYKKNPMVETLGTVLQLKQPLNTTRINAAEIESRVRELSKLAETTDKVKQGFWEEFETLQQQECKLLYSRKEGQRQENKNKNRYKNILPFDHTRVVLHDGDPNEPVSDYINANIIMPEFETKCNNSKPKKSYIATQGCLQNTVNDFWRMVFQENSRVIVMTTKEVERGKSKCVKYWPDEYALKEYGVMRVRNVKESAAHDYTLRELKLSKVGQGNTERTVWQYHFRTWPDHGVPSDPGGVLDFLEEVHHKQESIMDAGPVVVHCS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

75 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Protein-tyrosine phosphatase 1D, Protein-tyrosine phosphatase 2C, PTP-1D, PTP-2C, PTP2C, PTPN11, SH-PTP2, SH-PTP3, SHP-2, SHPTP2, Tyrosine-protein phosphatase non-receptor type 11

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-SHP2 Antibody (A93012)

Western blot analysis of extracts from normal (control) and SHP2 knockout (KO) 293T cells, using Anti-SHP2 Antibody (A93012) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Western Blot - Anti-SHP2 Antibody (A93012)

Western blot analysis of extracts of various cell lines, using Anti-SHP2 Antibody (A93012) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Immunofluorescence - Anti-SHP2 Antibody (A93012)

Immunofluorescence analysis of C6 cells using Anti-SHP2 Antibody (A93012) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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Western Blot - Anti-SHP2 Antibody (A93012)

Immunoprecipitation analysis of 300µg extracts of MCF7 cells using 3µg of Anti-SHP2 Antibody (A93012). This Western blot was performed on the immunoprecipitate using Anti-SHP2 Antibody (A93012) at a dilution of 1:2000.

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