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Anti-SGK1 Antibody (A12861)

Western Blot - Anti-SGK1 Antibody (A12861) - Antibodies.com Immunohistochemistry - Anti-SGK1 Antibody (A12861) - Antibodies.com Immunofluorescence - Anti-SGK1 Antibody (A12861) - Antibodies.com
$355
Product Datasheet

Rabbit polyclonal antibody to SGK1 for WB, IHC and ICC/IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-SGK1 Antibody
Description
Rabbit polyclonal antibody to SGK1.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 260-431 of human SGK1 (NP_005618.2).
Sequence
TPEYLAPEVLHKQPYDRTVDWWCLGAVLYEMLYGLPPFYSRNTAEMYDNILNKPLQLKPNITNSARHLLEGLLQKDRTKRLGAKDDFMEIKSHVFFSLINWDDLINKKITPPFNPNVSGPNDLRHFDPEFTEEPVPNSIGKSPDSVLVTASVKEAAEAFLGFSYAPPTDSFL
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
45 - 60 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Serine/threonine-protein kinase Sgk1, Serum/glucocorticoid-regulated kinase 1, SGK
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-SGK1 Antibody (A12861) - Antibodies.comEnlarge Image

Western Blot - Anti-SGK1 Antibody (A12861)

Western blot analysis of extracts of various cell lines, using Anti-SGK1 Antibody (A12861) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry - Anti-SGK1 Antibody (A12861) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-SGK1 Antibody (A12861)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-SGK1 Antibody (A12861) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescence - Anti-SGK1 Antibody (A12861) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-SGK1 Antibody (A12861)

Immunofluorescence analysis of L929 cells using Anti-SGK1 Antibody (A12861) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Publishing research using Anti-SGK1 Antibody (A12861)? Please let us know so that we can list the citation on this page.

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Proteins predicted to interact with SGK1

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
99.9% Relevancy Score
99.2% Relevancy Score
98.8% Relevancy Score
97.9% Relevancy Score
97.5% Relevancy Score
96.7% Relevancy Score
96.6% Relevancy Score
95.2% Relevancy Score
95% Relevancy Score