Anti-Serum Response Factor Antibody [PCRP-SRF-1F7] (A277815)

Mouse monoclonal [PCRP-SRF-1F7] antibody to Serum Response Factor for ELISA, IF, WB, IP, Flow Cytometry and IHC-P.

Alternative Formats and ConjugatesAlternative Formats

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 4-6 business days.
Name
Anti-Serum Response Factor Antibody [PCRP-SRF-1F7]
Description
Mouse monoclonal [PCRP-SRF-1F7] antibody to Serum Response Factor.
Specificity
Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF-binding sites are also constitutive promotor elements in many muscle-specific promotors. At the c-Fos SRE, formation of a ternary complex containing SRF and its accessory protein p62TCF appears to be important for signal transduction. Two related Ets domain proteins, Elk-1 and SRF accessory protein-1 (SAP-1), have DNA binding properties identical to that of p62TCF. Elk-1 and SAP-1 contain two homologous regions of which the two amino-terminal regions, the Ets domain (box A) and the B box, mediate ternary complex formation with SRF. The third homologous region, the C box located toward the C-terminus of the proteins, contains conserved consensus phosphorylation sites for MAP kinases.
Applications
ELISA, IF, WB, IP, Flow Cytometry, IHC-P
Dilutions
IF: 1-2 µg/ml, WB: 1-2 µg/ml, IP: 1-2µg / 100-500µg proteins, FC: 1-2 µg/million cells, IHC-P: 1-2 µg/ml
Reactivity
Human
Immunogen
Recombinant full-length human SRF protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
PCRP-SRF-1F7
Isotype
IgG2b
Conjugate

Unconjugated

Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Molecular Weight
40-67 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
General Notes
This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-Serum Response Factor Antibody [PCRP-SRF-1F7] - BSA and Azide free (A278403).
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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