Anti-SERT Antibody (A104336)

Immunofluorescent analysis of a rat brain section stained with Anti-SERT Antibody (green) and counterstained with Anti-Tyrosine hydroxylase Antibody (red). The axons of serotoninergic neurons course throughout the section but are clearly distinct from the chatacholinergic processes and cell bodies revealed with the Anti-Tyrosine hydroxylase Antibody. The blue stain reveals DNA in cell nuclei.

Western blot analysis of different tissue and cell lysates using Anti-SERT Antibody (1:1,000 | green). Left: [1] protein standard, [2] rat brain caudate/putmen region, [3] rat brain striatum region and [4] mouse brain. The prominent band at ~68kDa corresponds to SERT protein, and lower bands are likely are proteolytic products of SERT. Right: [5] protein standard, [6] non-transfected HEK293 cells and [7] HEK293 cells transfected with an expression construct containing a Myc-DDK tagged full length human SERT cDNA. The bands between 50-75kDa mark in the transfected cells demonstrate overexpression of SERT, and the band at about 150kDa presumably corresponds to an aggregated form of SERT. The blot was probed with Anti-GAPDH Antibody (1:5,000 | red), which reveals the single band at ~37kDa in both transfected and non-transfected cells. Note that HEK293 cells express SERT endogenously (7).