Anti-SDHB Antibody [SDHB/2382] - BSA and Azide free
Description
Mouse monoclonal [SDHB/2382] antibody to SDHB.
Specificity
Succinate dehydrogenase (SDH) is Complex II in the mitochondria, vital for mitochondrial electron transport, as well as Krebs cycle function.Four subunits comprise the SDH protein complex: a flavochrome subunit (SDHA), an iron-sulfur protein (SDHB) and two membrane-bound subunits (SDHC and SDHD) anchored to the inner mitochondrial membrane. The SDH complex functions as a tumor suppressor. Loss of any subunit proteins lead to destabilization of the complex and tumor formation.Antibody to SDHB is helpful in the identification of phaeochromocytomas, paragangliomas and GIST.
Applications
WB
Dilutions
WB: 1-2 µg/ml
Reactivity
Human
Immunogen
Recombinant fragment, around amino acids 165-273, of human SDHB protein. The exact sequence is proprietary.
Host
Mouse
Clonality
Monoclonal
Clone ID
SDHB/2382
Isotype
IgG2b
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A/G chromatography.
Concentration
1 mg/ml
Molecular Weight
32 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
SDS-PAGE analysis of Anti-SDHB Antibody [SDHB/2382] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.
Anti-SDHB Antibody [SDHB/2382] - BSA and Azide free (A253128)
Analysis of protein array containing more than 19,000 full-length human proteins using Anti-SDHB Antibody [SDHB/2382]. Z-Score and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target; a MAb is considered to be specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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