SDHB expression in HEK293 (A), Jurkat (B) and HeLa (C) cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-SDHB Antibody (A84115) at 0.1µg/ml and detected by chemiluminescence.
SDHB expression in Human Kidney analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by heating in citrate buffer, pH 6. Staining was performed with Anti-SDHB Antibody (A84115) at 6µg/ml and revealed with horseradish peroxidase (HRP).
Negative control for SDHB expression in Human Kidney analyzed by immunohistochemistry. Tissue was paraffin-embedded, and staining procedure was performed in the absence of primary antibody.
SDHB expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-SDHB Antibody (A84115) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and plasma membrane staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
SDHB expression in A431 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-SDHB Antibody (A84115) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.
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