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Anti-SARS-CoV-2 Spike Glycoprotein S1 Antibody (A309131)

Western Blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 Antibody (A309131) - Antibodies.com ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 Antibody (A309131) - Antibodies.com Immunofluorescence - Anti-SARS-CoV-2 Spike Glycoprotein S1 Antibody (A309131) - Antibodies.com Western Blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 Antibody (A309131) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to SARS-CoV-2 Spike Glycoprotein S1 for WB, ICC/IF, IP and Dot Blot.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-8 business days.

  • Specifications

Name
Anti-SARS-CoV-2 Spike Glycoprotein S1 Antibody
Description
Rabbit polyclonal antibody to SARS-CoV-2 Spike Glycoprotein S1.
Applications
WB, ICC/IF, IP, Dot Blot
Dilutions
DB: 1:500-1:2,000, ELISA: 1:20,000-1:80,000, WB: 1:500-1:1,000, ICC/IF: 1:100-1:500, IP: 1:2,000-1:10,000
Reactivity
SARS-CoV-2
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 11-682 of coronavirus Spike S1 (YP_009724390.1).
Sequence
VSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPR
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
110 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (4)

Western Blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 Antibody (A309131) - Antibodies.comEnlarge Image

Western Blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 Antibody (A309131)

Western blot analysis of extracts of 293T cells, using Anti-SARS-CoV-2 Spike Glycoprotein S1 Antibody (A309131) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 Antibody (A309131) - Antibodies.comEnlarge Image

Anti-SARS-CoV-2 Spike Glycoprotein S1 Antibody (A309131)

Anti-SARS-CoV-2 Spike Glycoprotein S1 Antibody (A309131) can bind immobilized recombinant SARS-COV-2 Spike S1 protein at 1 µg/ml (100 µl/well) with a linear range of 0.78-50ng/ml.
Immunofluorescence - Anti-SARS-CoV-2 Spike Glycoprotein S1 Antibody (A309131) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-SARS-CoV-2 Spike Glycoprotein S1 Antibody (A309131)

Immunofluorescence analysis of 293T cells transfected with SARS-CoV-2 Spike S1 fusion protein (top left) and untreated 293T cells (top right) using Anti-SARS-CoV-2 Spike Glycoprotein S1 Antibody (A309131) at a dilution of 1:400 (40x lens). DAPI was used to stain the cell nuclei (blue).
Western Blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 Antibody (A309131) - Antibodies.comEnlarge Image

Western Blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 Antibody (A309131)

Immunoprecipitation analysis of 300µg extracts of 293T cells using 3µg of Anti-SARS-CoV-2 Spike Glycoprotein S1 Antibody (A309131). This Western blot was performed on the immunoprecipitate using Anti-SARS-CoV-2 Spike Glycoprotein S1 Antibody (A309131) at a dilution of 1:10000.
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