Western Blot - Anti-S6K1 (phospho Thr389) Antibody [ARC55792] (A309436)
Western blot analysis of NIH/3T3, using Anti-S6K1 (phospho Thr389) Antibody [ARC55792] (A309436) at 1:8,000 dilution. NIH/3T3 cells were treated by λ-PP mixed solution (1ul) at 30°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Western Blot - Anti-S6K1 (phospho Thr389) Antibody [ARC55792] (A309436)
Western blot analysis of C6, using Anti-S6K1 (phospho Thr389) Antibody [ARC55792] (A309436) at 1:8,000 dilution. C6 cells were treated by λ-PP mixed solution (1ul) at 30°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
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