S100A4 expression in Peripheral blood mononuclear cells lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-S100A4 Antibody (A83734) at 0.05µg/ml and detected by chemiluminescence.
S100A4 expression in Human Small Intestine analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-S100A4 Antibody (A83734) at 2µg/ml and revealed with horseradish peroxidase (HRP).
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