S100 beta expression in Human Olfactory Bulb (A) and Cerebellum (B) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-S100 beta Antibody (A85168) at 1µg/ml and detected by chemiluminescence.
S100 beta expression in Human Cortex analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-S100 beta Antibody (A85168) at 5µg/ml and revealed with alkaline phosphatase (AP).
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