Anti-RYR2 Antibody (A329818)

Name: Anti-RYR2 Antibody
Brand: Antibodies.com
SKU: A329818
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to RYR2 for WB, IHC-P, ICC/IF and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-RYR2 Antibody

Description

Rabbit polyclonal antibody to RYR2.

Applications

WB, IHC-P, ICC/IF, ELISA

Positive Control

Mouse heart.

Dilutions

WB: 1:500-1:1,000, IHC-P: 1:100-1:500, ICC/IF: 1:50-1:200, ELISA: 1 µg/ml (starting concentration)

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to amino acids 1411-1510 of human RYR2.

Sequence

ADDRDDYDFLMQTSTYYYSVRIFPGQEPANVWVGWITSDFHQYDTGFDLDRVRTVTVTLGDEKGKVHESIKRSNCYMVCAGESMSPGQGRNNNGLEIGCV

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

565 kDa

Predicted MW

565 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% ProClin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Cardiac muscle ryanodine receptor, Cardiac muscle ryanodine receptor-calcium release channel, hRYR-2, Ryanodine receptor 2, RYR-2, Type 2 ryanodine receptor

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-RYR2 Antibody (A329818)

RYR2 expression in Mouse heart lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-RYR2 Antibody (A329818) at a dilution of 1:1000 and detected with chemiluminescence.

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Immunohistochemistry - Anti-RYR2 Antibody (A329818)

RYR2 expression in rat heart tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved with 10 mM citrate buffer, pH 6.0, under high pressure. Staining was performed with Anti-RYR2 Antibody (A329818) at a dilution of 1:300.

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Immunohistochemistry - Anti-RYR2 Antibody (A329818)

RYR2 expression in human heart tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved with 10 mM citrate buffer, pH 6.0, under high pressure. Staining was performed with Anti-RYR2 Antibody (A329818) at a dilution of 1:300.

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Immunofluorescence - Anti-RYR2 Antibody (A329818)

RYR2 expression in 293T cells analyzed by immunofluorescence. Staining was performed with Anti-RYR2 Antibody (A329818) at a dilution of 1:200 followed by Cy3-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).

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