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Ruplizumab Biosimilar - Anti-TNFSF5 Antibody - BSA and Azide free (A318914)

ELISA - Anti-TNFSF5 Humanized Antibody [Ruplizumab Biosimilar] - Azide free (A318914) - Antibodies.com Flow Cytometry - Anti-TNFSF5 Humanized Antibody [Ruplizumab Biosimilar] - Azide free (A318914) - Antibodies.com
Recombinant Antibody
$285
Product Datasheet

Recombinant humanized monoclonal antibody to TNFSF5 for ELISA and Flow Cytometry.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Ruplizumab Biosimilar - Anti-TNFSF5 Antibody - BSA and Azide free
Description
Recombinant humanized monoclonal antibody to TNFSF5.
Applications
ELISA, Flow Cytometry
Dilutions
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reactivity
Human
Host
Humanized
Clonality
Monoclonal
Isotype
IgG1
Conjugate

Unconjugated

Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Synonyms
CD154, CD40 ligand, CD40-L, CD40L, CD40LG, T-cell antigen Gp39, TNF-related activation protein, TRAP, Tumor necrosis factor ligand superfamily member 5
Suitable Secondaries
See all Anti-Human IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

ELISA - Anti-TNFSF5 Humanized Antibody [Ruplizumab Biosimilar] - Azide free (A318914) - Antibodies.comEnlarge Image

Ruplizumab Biosimilar - Anti-TNFSF5 Antibody - BSA and Azide free (A318914)

ELISA plates were pre-coated with 2 µg/ml (100 µl/well) Recombinant Human TRAP/CD40L Protein (Fc Tag) (A318295) which can bind Anti-TNFSF5 Humanized Antibody [Ruplizumab Biosimilar] - Azide free (A318914) in a linear range of 0.64-400 µg/ml. In order to specifically detect Anti-TNFSF5 Humanized Antibody [Ruplizumab Biosimilar] - Azide free (A318914), Mouse Anti-Human IgG Fab Antibody was used as detection antibody.
Flow Cytometry - Anti-TNFSF5 Humanized Antibody [Ruplizumab Biosimilar] - Azide free (A318914) - Antibodies.comEnlarge Image

Flow Cytometry - Ruplizumab Biosimilar - Anti-TNFSF5 Antibody - BSA and Azide free (A318914)

HEK293 cell line transfected with irrelevant protein (B) and human CD40L (A) were surface stained with Anti-TNFSF5 Humanized Antibody [Ruplizumab Biosimilar] - Azide free (A318914) at 1 µg/ml followed by Anti-Human IgG Antibody (Alexa 488).
Publishing research using Ruplizumab Biosimilar - Anti-TNFSF5 Antibody - BSA and Azide free (A318914)? Please let us know so that we can list the citation on this page.

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