Rabbit monoclonal [ARC1586] antibody to RPA32/RPA2 (phospho Thr21).
Applications
WB
Dilutions
WB: 1:500-1:2,000
Reactivity
Human, Rat
Immunogen
A synthetic phosphorylated peptide around T21 of human RPA2 (P15927).
Sequence
GYTQS
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC1586
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
32 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
REPA2, Replication factor A protein 2, Replication protein A 32 kDa subunit, Replication protein A 34 kDa subunit, RF-A protein 2, RP-A p32, RP-A p34, RPA32, RPA34
Western Blot - Anti-RPA32/RPA2 (phospho Thr21) Antibody [ARC1586] (A306163)
Western blot analysis of extracts of C6 cells, using Anti-RPA32/RPA2 (phospho Thr21) Antibody [ARC1586] (A306163) at 1:1,000 dilution. C6 cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 10s.
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