Anti-RPA2 Antibody (A329813)

Name: Anti-RPA2 Antibody
Brand: Antibodies.com
SKU: A329813
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to RPA2 for WB, IHC-P, ICC/IF and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-RPA2 Antibody

Description

Rabbit polyclonal antibody to RPA2.

Applications

WB, IHC-P, ICC/IF, ELISA

Positive Control

HeLa.

Dilutions

WB: 1:1,000-1:5,000, IHC-P: 1:100-1:500, ICC/IF: 1:100-1:500

Reactivity

Human

Immunogen

A recombinant protein corresponding to amino acids 1-270 of human RPA2.

Sequence

MWNSGFESYGSSSYGGAGGYTQSPGGFGSPAPSQAEKKSRARAQHIVPCTISQLLSATLVDEVFRIGNVEISQVTIVGIIRHAEKAPTNIVYKIDDMTAAPMDVRQWVDTDDTSSENTVVPPETYVKVAGHLRSFQNKKSLVAFKIMPLEDMNEFTTHILEVINAHMVLSKANSQPSAGRAPISNPGMSEAGNFGGNSFMPANGLTVAQNQVLNLIKACPRPEGLNFQDLKNQLKHMSVSSIKQAVDFLSNEGHIYSTVDDDHFKSTDAE

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

35 kDa

Predicted MW

29 kDa, 30 kDa, 38 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% ProClin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

REPA2, Replication factor A protein 2, Replication protein A 32 kDa subunit, Replication protein A 34 kDa subunit, RF-A protein 2, RP-A p32, RP-A p34, RPA32, RPA34

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-RPA2 Antibody (A329813)

RPA2 expression in HeLa cell lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-RPA2 Antibody (A329813) at a dilution of 1:2000 and detected with chemiluminescence.

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Immunohistochemistry - Anti-RPA2 Antibody (A329813)

RPA2 expression in human colon tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved with 10 mM citrate buffer, pH 6.0, under high pressure. Staining was performed with Anti-RPA2 Antibody (A329813) at a dilution of 1:100.

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Immunofluorescence - Anti-RPA2 Antibody (A329813)

RPA2 expression in U2OS cells analyzed by immunofluorescence. Staining was performed with Anti-RPA2 Antibody (A329813) at a dilution of 1:200 followed by Cy3 Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).

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