Anti-ROCK1 Antibody (A12527)

Name: Anti-ROCK1 Antibody
Brand: Antibodies.com
SKU: A12527
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to ROCK1 for WB, ICC/IF, IP and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-ROCK1 Antibody

Description

Rabbit polyclonal antibody to ROCK1.

Applications

WB, ICC/IF, IP, ELISA

Positive Control

Jurkat, HeLa, 293T, BT-474, Mouse liver, Rat liver.

Dilutions

WB: 1:500-1:2,000, IP: 0.5µg-4µg antibody for 200µg-400µg extracts of whole cells, ELISA: 1 µg/ml (starting concentration)

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant fusion protein corresponding to amino acids 400-660 of human ROCK1.

Sequence

YSNRRYLSSANPNDNRTSSNADKSLQESLQKTIYKLEEQLHNEMQLKDEMEQKCRTSNIKLDKIMKELDEEGNQRRNLESTVSQIEKEKMLLQHRINEYQRKAEQENEKRRNVENEVSTLKDQLEDLKKVSQNSQLANEKLSQLQKQLEEANDLLRTESDTAVRLRKSHTEMSKSISQLESLNRELQERNRILENSKSQTDKDYYQLQAILEAERRDRGHDSEMIGDLQARITSLQEEVKHLKHNLEKVEGERKEAQDMLN

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

158 kDa

Predicted MW

158 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

p160 ROCK-1, p160ROCK, Renal carcinoma antigen NY-REN-35, Rho-associated protein kinase 1, Rho-associated, coiled-coil-containing protein kinase 1, Rho-associated, coiled-coil-containing protein kinase I, ROCK-I

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-ROCK1 Antibody (A12527)

ROCK1 expression in various lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-ROCK1 Antibody (A12527) at a dilution of 1:1000 and detected with chemiluminescence.

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Western Blot - Anti-ROCK1 Antibody (A12527)

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Immunofluorescence - Anti-ROCK1 Antibody (A12527)

ROCK1 expression in Human esophageal cancer tissue analyzed by immunofluorescence. Staining was performed with Anti-ROCK1 Antibody (A12527) at a dilution of 1:100 followed by Cy3-conjugated Goat anti-Rabbit IgG (H+L)secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).