Anti-RNA polymerase II CTD repeat YSPTSPS (phospho Ser5) Antibody (A88928)

Western blot analysis of extracts of various cell lines, using Anti-RNA polymerase II CTD repeat YSPTSPS (phospho Ser5) Antibody (A88928) at 1:1,000 dilution. MCF7 and C2C12 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-RNA polymerase II CTD repeat YSPTSPS (phospho Ser5) Antibody (A88928) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-RNA polymerase II CTD repeat YSPTSPS (phospho Ser5) Antibody (A88928) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-RNA polymerase II CTD repeat YSPTSPS (phospho Ser5) Antibody (A88928) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.