Reconstitute with 100µl of sterile double-distilled water to bring antibody to 1mg/ml concentration. Gently shake to solubilize completely. Do not vortex!
Formulation
Lyophilized from 25mM Histadine, pH 6.2, with 8% Sucrose and 0.01% Tween80.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
SDS-PAGE analysis of Rituximab Biosimilar - Anti-CD20 Antibody - Low endotoxin, Azide free (A323702) under reducing (R) conditions confirms that the purity of this antibody is greater than 95%.
SEC-HPLC analysis of Rituximab Biosimilar - Anti-CD20 Antibody - Low endotoxin, Azide free (A323702) confirms that the purity of this antibody is 98.97%.
Raji cells were stained with Rituximab Biosimilar - Anti-CD20 Antibody - Low endotoxin, Azide free (A323702) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC50 = 1.334 µg/ml.
Rituximab Biosimilar - Anti-CD20 Antibody - Low endotoxin, Azide free (A323702) induced CDC activity was evaluated using Raji Cell. The max lysis rate was approximately 74%.
Rituximab Biosimilar - Anti-CD20 Antibody - Low endotoxin, Azide free (A323702) induced ADCC activity was evaluated using Raji Cell. The max Lysis rate was approximately 35%.
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