This antibody recognises the red fluorescent protein DsRed isolated from the mushroom coral Discosoma striata. The protein is also known as RFP and dsdrFP583. DsRed is a ~27 kDa protein that is optimally excited at a wavelength of 558 nm and emits light at 583 nm. In contrast to most other fluorescent proteins, which are monomers, DsRed tends to appear in a tetramer configuration.
Applications
WB
Dilutions
WB: 0.5 - 4.0 µg/ml
Immunogen
E. coli derived recombinant red fluorescent protein.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Concentration
500 µg/ml
Molecular Weight
Approximately 27 kDa on recombinant RFP. The observed band size is approximately 32 kDa on SDS PAGE gel.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 30% Glycerol, 0.5% BSA, 5mM EDTA, and 0.03% ProClin 300.
Storage
Store undiluted at -20°C. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Rabbit anti red fluorescent protein antibody recognizes the red fluorescent protein DsRed isolated from the mushroom coral Discosoma striata. The protein is also known as RFP and dsdrFP583 (Day and Davidson 2009). DsRed is a ~27 kDa protein that is optimally excited at a wavelength of 558 nm and emits light at 583 nm (Day and Davidson 2009). In contrast to most other fluorescent proteins, which are monomers, DsRed tends to appear in a tetramer configuration.
