Rabbit polyclonal antibody to RFA2 (phospho Thr21).
Specificity
This antibody detects endogenous levels of RFA2 only when phosphorylated at Thr21.
Applications
WB, IHC, ELISA
Dilutions
WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:1000
Reactivity
Human, Mouse
Immunogen
Synthetic peptide derived from human RFA2 around the phosphorylation site of Thr21 (amino acids 10-59).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
29kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
REPA2, Replication factor A protein 2, Replication protein A 32 kDa subunit, Replication protein A 34 kDa subunit, RF-A protein 2, RP-A p32, RP-A p34, RPA32, RPA34
Western Blot - Anti-RFA2 (phospho Thr21) Antibody (A94101)
Western blot analysis of lysates from HeLa cells treated with Adriamycin 0.5ug/ml 24h using Anti-RFA2 (phospho Thr21) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Anti-RFA2 (phospho Thr21) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
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