Anti-Retinoblastoma (phospho Thr826) Antibody (A94558)

Name: Anti-Retinoblastoma (phospho Thr826) Antibody
Brand: Antibodies.com
SKU: A94558
Price: 190.0 USD
Availability: InStock

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$190

Rabbit polyclonal antibody to Retinoblastoma (phospho Thr826) for WB, IF and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-Retinoblastoma (phospho Thr826) Antibody

Description

Rabbit polyclonal antibody to Retinoblastoma (phospho Thr826).

Specificity

This antibody detects endogenous levels of Retinoblastoma only when phosphorylated at Thr826.

Applications

WB, IF, ELISA

Dilutions

WB: 1:500-1:1000, ELISA: 1:20000

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human Retinoblastoma around the phosphorylation site of Thr826 (amino acids 601-650).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing peptide.

Molecular Weight

106kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

p105-Rb, p110-RB1, pp110, pRb, RB1, Retinoblastoma-associated protein

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-Retinoblastoma (phospho Thr826) Antibody (A94558)

Western blot analysis of lysates from HepG2 cells treated with nocodazole 1µg/ml 16h using Anti-Retinoblastoma (phospho Thr826) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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Immunofluorescence - Anti-Retinoblastoma (phospho Thr826) Antibody (A94558)

Immunofluorescence analysis of COS7 cells using Anti-Retinoblastoma (phospho Thr826) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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Western Blot - Anti-Retinoblastoma (phospho Thr826) Antibody (A94558)

Western blot analysis of rat musle using Anti-Retinoblastoma (phospho Thr826) Antibody.

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Immunohistochemistry - Anti-Retinoblastoma (phospho Thr826) Antibody (A94558)

Immunohistochemical analysis of paraffin-embedded human brain using Anti-Retinoblastoma (phospho Thr826) Antibody 1:100 (4°C overnight). The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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