Recombinant rabbit monoclonal [RB1/2313R] antibody to Rb1.
Specificity
This antibody reacts with the hyper-phosphorylated as well as the un (under) phosphorylated form of the Rb protein. This antibody does not cross-react with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells.
Applications
IHC-P
Dilutions
IHC-P: 1-2 µg/ml
Reactivity
Human, Mouse
Immunogen
Recombinant full-length human Rb1 protein.
Host
Rabbit
Clonality
Monoclonal
Clone ID
RB1/2313R
Isotype
IgG
Conjugate
Unconjugated
Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Molecular Weight
105 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
SDS-PAGE analysis of Anti-Rb1 Antibody [RB1/2313R] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.
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