Anti-Rb (phospho Ser780) Antibody [ARC0096] (A308931)

Western blot analysis of extracts of C6 cells, using Anti-Rb (phospho Ser780) Antibody [ARC0096] (A308931) at 1:1,000 dilution. C6 cells were treated by 10% FBS at 37°C for 30 minutes after serum-starvation overnightThe secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry analysis of paraffin-embedded rat pancreas using Anti-Rb (phospho Ser780) Antibody [ARC0096] (A308931) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-Rb (phospho Ser780) Antibody [ARC0096] (A308931) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-Rb (phospho Ser780) Antibody [ARC0096] (A308931) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.