The antibody detects endogenous level of Rb only when phosphorylated at serine 780.
Applications
WB, IHC, IF
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of serine 780 (T-L-S(p)-P-I) derived from Human Rb.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Synonyms
p105-Rb, p110-RB1, pp110, pRb, RB1, Retinoblastoma-associated protein
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rb (Phospho-Ser780) Antibody #11132 (left) or the same antibody preincubated with blocking peptide (right).
![Western Blot - Anti-Rb (phospho Ser780) Antibody [ARC0096] (A308931) - Antibodies.com](https://cdn.antibodies.com/image/catalog/308/A308931_1.jpg?profile=product_alternative)
