Anti-Rb (phospho Ser608) Antibody (A11048)

Western blot analysis of extracts of Jurkat cells, using Anti-Rb (phospho Ser608) Antibody (A11048) at 1:2,000 dilution. Jurkat cells were treated by 10% FBS for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 30s.