Recombinant Anti-Rb Antibody [rRB1/8060] - BSA and Azide free (A316925)

Anti-Rb Antibody [rRB1/8060] - BSA and Azide free

Recombinant mouse monoclonal [rRB1/8060] antibody to Rb.

Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. Its epitope is localized between aa 703-772. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.

WB, IHC-P

HeLa, A431 or BT-20 cells. Human breast or colon carcinoma.

WB: 1-2 µg/ml, IHC: 1-2 µg/ml

Human, Mouse

Recombinant human Rb1 protein fragment of 283 amino acid residues.

Mouse

Monoclonal

rRB1/8060

kappa

Protein A/G chromatography.

1 mg/ml

105 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

p105-Rb, p110-RB1, pp110, pRb, RB1, Retinoblastoma-associated protein