Use 10µl of the suggested working dilution to label 106 cells in 100µl.
Immunogen
Horseradish peroxidase.
Host
Rat
Clonality
Monoclonal
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline.
Storage
Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Rat IgG1 negative control, a rat monoclonal raised against horseradish peroxidase, is recommended for use as a negative control to assess the level of non-specific binding of rat IgG1 test antibodies to the surface of human and mouse cells in flow cytometry.Test results have shown that this antibody is also suitable for use as a negative control with porcine and canine cells.
