Anti-RAP80 Antibody (A15614)

Name: Anti-RAP80 Antibody
Brand: Antibodies.com
SKU: A15614
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to RAP80 for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-RAP80 Antibody

Description

Rabbit polyclonal antibody to RAP80.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human UIMC1 (NP_057374.3).

Sequence

MPRRKKKVKEVSESRNLEKKDVETTSSVSVKRKRRLEDAFIVISDSDGEEPKEENGLQKTKTKQSNRAKCLAKRKIAQMTEEEQFALALKMSEQEAREVNSQEEEEEELLRKAIAESLNSCRPSDASATRSRPLATGPSSQSHQEKTTDS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

120 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-RAP80 Antibody (A15614)

Western blot analysis of extracts of various cell lines, using Anti-RAP80 Antibody (A15614) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Immunofluorescence - Anti-RAP80 Antibody (A15614)

Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using Anti-RAP80 Antibody (A15614). Green:GFP-RNF168 fusion protein expression for DNA damage marker. DAPI was used to stain the cell nuclei (blue). RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.

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