Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-Rab4 Antibody (A304983), at 1:150 for 12 hours at 4°C. The secondary antibody used was APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Membrane. Cytoplasm. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-Rab4 Antibody. (C) Composite. Heat Shocked at 42°C for 30 min.
Immunocytochemistry/Immunofluorescence analysis of human HaCaT cells, fixed in cold 100% methanol at -20°C for 10 minutes, using Anti-Rab4 Antibody (A304983), at 1:100 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at room temperature in dark. Localization: String nuclear and cytoplasmic staining.
Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution. The Primary Antibody used was Anti-Rab4 Antibody (A304983) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at room temperature. Localization: Epidermis (cell-cell border and cytoplasmic), hair follicles and muscle.
Western blot analysis of human Cervical cancer cell line (HeLa) lysate showing detection of Rab4 protein using Anti-Rab4 Antibody (A304983) at 1:1,000 for 2 hours at room temperature. Load: 15 µgprotein. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Donkey Anti-Rabbit IgG: HRP for 1 hour at room temperature.
Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-Rab4 Antibody (A304983), at 1:150 for 12 hours at 4°C. The secondary antibody used was R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Membrane. Cytoplasm. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-Rab4 Antibody. (C) Composite. Heat Shocked at 42°C for 30 min.
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