Anti-RAB35 Antibody (A12986)

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This product is discontinued

Anti-RAB35 Antibody (A12986) has been discontinued and is no longer available.

View all RAB35 Antibodies.

Specifications

Name

Anti-RAB35 Antibody

Description

Rabbit polyclonal antibody to RAB35.

Applications

Dilutions

WB: 1:500-1:2000

Reactivity

Human, Mouse

Immunogen

A synthetic peptide of human RAB35.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

GTP-binding protein RAY, RAB1C, Ras-related protein Rab-1C, Ras-related protein Rab-35, RAY

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-RAB35 Antibody (A12986)

Western blot analysis of extracts of various cell lines, using TST antibody (A10542) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

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Immunoprecipitation - Anti-RAB35 Antibody (A12986)

Immunoprecipitation analysis of 200µg extracts of A-549 cells, using 3µg TST antibody (A10542). Western blot was performed from the immunoprecipitate using TST antibody (A10542) at a dilition of 1:1000.