Pumilio 2 expression in HEK293 cell lysate analyzed by western blot. Cells were transfected to overexpress FLAG tagged Pumilio 2 an lysed in RIPA buffer. Primary incubation was performed for 1 hour on 10µg protein per lane with Anti-Pumilio 2 Antibody (A84071) at 0.5µg/ml (Lane A) or Anti-FLAG at 1:3000 dilution (Lane C) and detected with chemiluminescence. Control: Mock-transfected HEK293 cells incubated with Anti-Pumilio 2 Antibody (A84071) at 1mg/ml (Lane B).
Pumilio 2 expression in Human Testis analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-Pumilio 2 Antibody (A84071) at 10µg/ml and revealed with alkaline phosphatase (AP).
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