Immunohistochemistry analysis of human colon carcinoma, fixed in formalin. The Primary Antibody used was Anti-PUMA Antibody (A305172) at 1:10,000 for 12 hours at 4°C. The secondary antibody used was Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at room temperature. Counterstain: Methyl Green at 200uL for 2 min at room temperature.