Anti-PUMA Antibody (A283911)

Anti-PUMA Antibody

Rabbit polyclonal antibody to PUMA.

This antibody recognises the p53 - upregulated modulator of apoptosis, also known as Bcl-2 binding component 3, JFY-1 or Bcl-2. PUMA is a 193 amino acid ~20 kDa pro-apoptotic member of the BCL-2 family.Bcl-2 binding component 3 is localized to the mitochondria, binds BCL-2 and induces cytochrome C release and apoptosis in response to p53. Four isoforms of PUMA have been described due to alternative splicing. Rabbit anti PUMA antibody is expected to recognize the canonical isoform 1, also known as PUMA alpha, it is not expected to recognize any of the other isoforms of PUMA.

WB, IHC-P

IHC-P 1: 10 µg/ml, WB: 2 µg/ml - 4 µg/ml

Human, Mouse

Synthetic peptide sequence ARARQEGSSPEPVEG corresponding to the N-terminus of human Bcl-2 binding component 3.

ARARQEGSSPEPVEG

Rabbit

Polyclonal

IgG

Approximately 23 kDa in K562 cell lysates.

Liquid

Supplied in Phosphate Buffered Saline with 0.02% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rabbit anti PUMA antibody recognizes the p53 - upregulated modulator of apoptosis, also known as Bcl-2 binding component 3, JFY-1 or Bcl-2. PUMA is a 193 amino acid ~20 kDa pro-apoptotic member of the BCL-2 family.Bcl-2 binding component 3 is localized to the mitochondria, binds BCL-2 and induces cytochrome C release and apoptosis in response to p53 (Follis et al. 2013). Four isoforms of PUMA have been described due to alternative splicing. Rabbit anti PUMA antibody is expected to recognize the canonical isoform 1, also known as PUMA alpha, it is not expected to recognize any of the other isoforms of PUMA.

BBC3, Bcl-2-binding component 3, isoforms 1/2, JFY-1, p53 up-regulated modulator of apoptosis