Anti-PU.1/Spi1 Antibody (A83352)

Name: Anti-PU.1/Spi1 Antibody
Brand: Antibodies.com
SKU: A83352
Price: 495.0 USD
Availability: InStock

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$495

Goat polyclonal antibody to PU.1/Spi1 for ELISA, WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 3-5 business days.

Specifications

Name

Anti-PU.1/Spi1 Antibody

Description

Goat polyclonal antibody to PU.1/Spi1.

Applications

ELISA, WB, IHC

Dilutions

ELISA: 1:32,000, WB: 0.03-0.1µg/ml, IHC: 3.75µg/ml

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the internal region of human PU.1/Spi1.

Sequence

C-DLYQRQTHEYY

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

40 kDa

Predicted MW

31.2kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

31 kDa-transforming protein, SPI1, Transcription factor PU.1

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-PU.1/Spi1 Antibody (A83352)

PU.1/Spi1 expression in Daudi cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-PU.1/Spi1 Antibody (A83352) at 0.03µg/ml and detected by chemiluminescence.

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Immunohistochemistry - Anti-PU.1/Spi1 Antibody (A83352)

PU.1/Spi1 expression in Human Spleen analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-PU.1/Spi1 Antibody (A83352) at 3.75µg/ml and revealed with alkaline phosphatase (AP).

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Immunohistochemistry - Anti-PU.1/Spi1 Antibody (A83352)

PU.1/Spi1 expression in Human Colon analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-PU.1/Spi1 Antibody (A83352) at 3.75µg/ml and revealed with alkaline phosphatase (AP).

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