Recombinant Anti-PU.1 Antibody [rPU1/2146] (A277922)

Anti-PU.1 Antibody [rPU1/2146]

Recombinant mouse monoclonal [rPU1/2146] antibody to PU.1.

PU.1 is a member of the ETS family of transcription factors and is important for normal B-cell development. It is expressed in the myeloid lineage and in immature as well as mature B-lymphocytes, with the exception of plasma cells. PU.1 is expressed in germinal center B-cells and mantle B-cells. Various lymphomas are also positive for this marker. It is essential during early B-cell differentiation. The absence of PU.1 results in total block of B-cell development at the pre-pro stage. PU.1 plays a key role in normal myeloid differentiation, and regulates the expression of immunoglobulin and other genes that are important for B-cell development.

Flow Cytometry, IF, WB, IHC-P

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml

Human

Recombinant fragment, around amino acids 16-170, of human PU.1 protein. The exact sequence is proprietary.

Mouse

Monoclonal

rPU1/2146

IgG1

kappa

Protein A/G chromatography.

200 µg/ml

40 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-PU.1 Antibody [rPU1/2146] - BSA and Azide free (A278510).

31 kDa-transforming protein, SPI1, Transcription factor PU.1